Antioxidant Properties of Active Fraction Extract Derived from Yellow-Red Pigment Produced by the Marine Sponge-Associated Bacterium Bacillus haikouensis AGS112 and Identification of Related Compounds

Sponge-associated bacteria can produce bioactive compounds similar to the host. Here, investigation of antioxidant properties yellow-red pigment produced by sponge-associated bacterium AGS112 was conducted using in vitro and vivo analysis. The 16S rRNA gene sequence showed has closest similarity with Bacillus haikouensis C-89 (99%). crude extract contained both phenolic flavonoid. peaks occurred at 412 nm 664 nm, which indicated as presence carotenoids flavonoids, respectively. active fraction obtained through bio-autography TLC, had more potent activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) compared an IC50 value 68.62±0.59 µg/ml 198.88±1.66 µg/ml, Moreover, concentration 35 could better enhance viability Schizosaccharomyces pombe achieve highest expression sod1 ctt1 genes. Metabolite profiling LC-MS analysis confirmed contains eudesmin artelastin that might be contributed antioxidants. These results suggest B. potency used candidate for natural